Shanghai Ruifu Chemical Co., Ltd. is the leading manufacturer and supplier of Fluorescamine (CAS: 38183-12-9) with high quality. We can provide COA, worldwide delivery, small and bulk quantities available. Please contact: alvin@ruifuchem.com
| Chemical Name | Fluorescamine |
| Synonyms | 4-Phenylspiro[furan-2(3H),1'-phthalan]-3,3'-dione |
| CAS Number | 38183-12-9 |
| CAT Number | RF-PI2189 |
| Stock Status | In Stock, Production Scale Up to Tons |
| Molecular Formula | C17H10O4 |
| Molecular Weight | 278.26 |
| Stability | Hygroscopic |
| Store Under Inert Gas | Store Under Inert Gas |
| Solubility | Soluble in Acetone, Ethanol, Chloroform, Dimethylsulfoxide and Acetonitrile. Slightly Soluble in Water |
| Spectral Properties | Excitation (nm): 382, Emission (nm): 480, Absorbance (nm): 237 |
| Brand | Ruifu Chemical |
| Item | Specifications |
| Appearance | Light Yellow Powder |
| Purity / Analysis Method | >98.0% (HPLC) |
| Melting Point | 153.0~157.00℃ |
| Water (by Karl Fischer) | <0.50% |
| Total Impurities | <2.00% |
| Carbon by Elemental Analysis | 72.88~73.88% (Anhydrous) |
| Molar Extinction Coefficient | Diethyl Ether, 233.0 to 235.0nm |
| Identity by NMR (CDCl3) | Consistent |
| Solubility in Acetone | Colorless to Yellow, Clear, 50.00 mg/ml Pass |
| Test Standard | Enterprise Standard |
Package: Bottle, Aluminium foil bag, 25kg/Cardboard Drum, or according to customer's requirement
Storage Condition: Store in sealed containers at cool and dry place; Ventilation; 2~8℃; Protect from light and moisture; Nitrogen protection can extend shelf life
Fluorescamine (CAS: 38183-12-9) is a non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds. Reaction takes place under mild conditions. Low background due to hydrolysis. Analytical reagent. For fluorescent detection of amino acids, peptides and proteins. Fluorescamine plays an important role as a reagent for the detection of amines, peptides and proteins. It is also used as a substrate for measuring protease activity. It is utilized in the detection and quantitation of residual aminopenicillins and sulfonamides in honey by HPLC. It finds application in the determination of lisinopril in human plasma and urine. Adequately blocks newly generated amino termini in protein sequence analyses. Fluorescamine is a popular amine-fluorogenic reagent for determining protein concentrations in solutions and on gels. It is also used to analyze low molecular weight amines by TLC, HPLC and capillary electrophoresis. Fluorescamine is nonfluorescent but readily reacts with primary aliphatic amines, including those in peptides and proteins, to yield a strongly blue-green fluorescent adduct that can be excited by UV light. The amine adduct has absorption maximum at 385 nm and fluorescence maximum at 486 nm.