Shanghai Ruifu Chemical Co., Ltd. is the leading manufacturer and supplier of 5-Carboxytetramethylrhodamine, 5-TAMRA (CAS: 91809-66-4) with high quality. We can provide COA, worldwide delivery, small and bulk quantities available. Please contact: alvin@ruifuchem.com
| Chemical Name |
5-Carboxytetramethylrhodamine |
| Synonyms |
5-TAMRA |
| CAS Number |
91809-66-4 |
| CAT Number |
RF-PI2188 |
| Stock Status |
In Stock, Production Scale Up to Tons |
| Molecular Formula |
C25H22N2O5 |
| Molecular Weight |
430.45 |
| Spectral Properties |
Excitation Wavelength: 552 nm
Emission Wavelength: 578 nm |
| Solubility |
Soluble In DMSO |
| Source |
Synthetic |
| Category |
Fluorescent Reagents |
| Brand |
Ruifu Chemical |
| Item |
Specifications |
| Appearance |
Red to Dark Purple Powder to Soild (Visual Inspection) |
| Identity |
1H NMR, MS Conforms |
| Purity / Analysis Method |
>97.0% (HPLC at 254nm)
Column: XDB-C18
Flow Rate: 0.8mL/min
Detection: UV at 254nm
HPLC Elution Buffer: CH3CN:TFA (0.1%)=35:65 |
| Test Standard |
Enterprise Standard |
| Shelf Life |
12 Months From Manufacture Date if Stored Properly |
Package: Bottle, Aluminium foil bag, 25kg/Cardboard Drum, or according to customer's requirement
Storage Condition: Store in sealed containers at cool and dry place, Freeze (<-15℃), Minimize light exposure, Keep in dark place, Inert atmosphe, Protect from moisture
5-Carboxytetramethylrhodamine, 5-TAMRA (CAS: 91809-66-4), is a long-wavelength dye, fluorescent dye for DNA, peptides and proteins. Amine-reactive. Used for oligonucleotide labeling and automated DNA sequencing applications. TAMRA is often used as FRET acceptor for FAM fluorophore. Free 5(6)-TAMRA (ab145282) and 6-TAMRA (ab145338) isomer. 5-TAMRA N-succinimidyl ester (ab145433) precursor. 5-TAMRA, SE and its conjugates yield bright, pH-insensitive orange-red fluorescence with good photostability. 5-TAMRA is the purified single isomer of 5(6)-TAMRA. It is widely used to label peptides and proteins. It is preferred for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-TAMRA and 6-TAMRA might affect some biological properties of the underlying conjugates. Currently AAT Bioquest is using a proprietary process to synthesize isomer-free 5-TAMRA without the conventional chromatographic purification.