Biological Buffers; Ultra Pure Grade; Molecular Biology Grade
PIPES Free Acid CAS: 5625-37-6
PIPES Disodium Salt CAS: 76836-02-7
PIPES Monosodium Salt CAS: 10010-67-0
PIPES Sesquisodium Salt CAS: 100037-69-2
Chemical Name |
PIPES Monosodium Salt |
Synonyms |
PIPES.Na; PIPES 1Na; Piperazine-1,4-bis(2-Ethanesulfonic Acid) Monosodium Salt; Piperazine-N,N'-bis-(2-Ethanesulphonic Acid) Monosodium Salt; 1,4-Piperazinediethanesulfonic Acid Monosodium Salt; PIPES Sodium Salt |
CAS Number |
10010-67-0 |
CAT Number |
RF-PI1660 |
Stock Status |
In Stock, Production Scale Up to Tons |
Molecular Formula |
C8H17N2NaO6S2 |
Molecular Weight |
324.34 |
Melting Point |
300℃ |
Brand |
Ruifu Chemical |
Item |
Specifications |
Appearance |
White Crystalline Powder |
Purity / Analysis Method |
>99.0% (Titration, on Dried Basis) |
Useful pH Range |
6.1~7.5 |
Water (by Karl Fisher) |
≤1.00% |
Ignition Residue (Sulfate) |
≤0.50% |
Heavy Metals (as Pb) |
≤5ppm |
UV A260nm |
≤0.06 (0.1M in H2O) |
UV A280nm |
≤0.04 (0.1M in H2O) |
Solubility (2% in H20) |
Clear and Complete |
pKa |
6.8 (at 25℃) |
pH (2% in H2O) |
4.0~5.0 |
DNase |
Not detected |
RNase |
Not detected |
Protease |
Not detected |
Infrared Spectrometry |
Conforms to Structure |
Grade |
Ultra Pure Grade; Molecular Biology Grade |
Test Standard |
Enterprise Standard |
Usage |
Biological Buffer; Good's Buffer Component for Biological Research
|
Package: Bottle, Aluminium foil bag, 25kg/Cardboard Drum, or according to customer's requirement.
Storage Condition: Store in sealed containers at cool and dry place; Protect from light and moisture.
PIPES Monosodium Salt (CAS: 10010-67-0) is frequently used as a buffering agent in biochemistry. It is useful in cell culture work. It is an ethanesulfonic acid buffer developed by Good et al. in the 1960s. PIPES has a pKa near the physiological pH which makes it useful in cell culture work. It has been documented to minimize lipid loss when buffering glutaraldehyde histology in plant and animal tissues. Buffers can be prepared by adding a solution of base to PIPES free acid, titrating to the appropriate pH, or by mixing equimolar solutions of the monosodium salt and the disodium salt, titrating to the appropriate pH. Additional forms available:
PIPES Free Acid (CAS: 5625-37-6);
PIPES Sesquisodium Salt (CAS: 100037-69-2);
PIPES Monosodium Salt (CAS 10010-67-0);
PIPES Disodium Salt (CAS: 76836-02-7).