PIPES Free Acid CAS: 5625-37-6
PIPES Dipotassium Salt CAS 108321-27-3
PIPES Disodium Salt CAS: 76836-02-7
PIPES Monosodium Salt CAS: 10010-67-0
PIPES Sesquisodium Salt CAS: 100037-69-2
| Chemical Name | PIPES |
| Synonyms | PIPES Free Acid; 1,4-Piperazinediethanesulfonic Acid; 1,4-Piperazinebis(ethanesulfonic Acid); Piperazine-1,4-Bisethanesulfonic Acid; 2,2'-(Piperazine-1,4-diyl)diethanesulfonic Acid |
| CAS Number | 5625-37-6 |
| CAT Number | RF-PI1633 |
| Stock Status | In Stock, Production Scale Up to Tons |
| Molecular Formula | C8H18N2O6S2 |
| Molecular Weight | 302.37 |
| Melting Point | >300℃ (lit.) |
| Density | 1.4983 |
| Brand | Ruifu Chemical |
| Item | Specifications |
| Grade | Ultra Pure Grade |
| Appearance | White Crystalline Powder |
| Purity / Analysis Method | >99.5% (Titration) |
| Loss on Drying | <0.50% |
| Sulphated Ash | <0.10% |
| Useful pH Range | 6.1~7.5 |
| UV A260nm | ≤0.20 (0.5M in 1N NaOH) |
| UV A280nm | ≤0.20 (0.5M in 1N NaOH) |
| Solubility | Clear, Colorless Solution (5% 1N NaOH) |
| pKa (25°C) | 6.6~7.0 |
| Heavy Metals (as Pb) | <5ppm |
| Iron (Fe) | <5ppm |
| Arsenic (As) | <0.1ppm |
| Barium (Ba) | <5ppm |
| Bismuth (Bi) | <5ppm |
| Calcium (Ca) | <10ppm |
| Cadmium (Cd) | <5ppm |
| Cobalt (Co) | <5ppm |
| Chromium (Cr) | <5ppm |
| Copper (Cu) | <5ppm |
| Iron (Fe) | <5ppm |
| Potassium (K) | <50ppm |
| Lithium (Li) |
<5ppm |
| Magnesium (Mg) | <5ppm |
| Molybdenum (Mo) | <5ppm |
| DNase | Not Detected |
| RNase | Not Detected |
| Protease | Not Detected |
| Infrared Spectrum | Conforms to Structure |
| Test Standard | Enterprise Standard |
| Usage | Biological Buffer; Good's Buffer; Pharmaceutical Intermediates |
Package: Bottle, Aluminium foil bag, 25kg/Cardboard Drum, or according to customer's requirement.
Storage Condition: Store in sealed containers at cool and dry place; Protect from light and moisture.
PIPES (CAS: 5625-37-6) is used buffering agent in biochemistry, is a Good's buffer notable for having a pKa value similar to ordinary physiological pH. As such, PIPES free acid is frequently used as a buffer in biochemical research. PIPES is a zwitterionic, piperazinic buffer that is useful for a pH range of 6.1~7.5. PIPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. PIPES has wide variety of applications and is commonly used in cell culture media, in protein crystallization, as a running buffer in gel electrophoresis, and as an eluent in isoelectric focusing and chromatography. This buffer is capable of forming radicals and is therefore not suitable for redox reactions. It is suitable for use in the bicinchoninic acid (BCA) assay. Solubility of PIPES increases when the free acid is converted to the sodium salt.