Ruifu Chemical is the leading manufacturer of N-(4-Aminobenzoyl)-L-Glutamic Acid (H-4-ABZ-Glu-OH) (CAS: 4271-30-1) with high quality, production capacity 30 tons per month, intermediate of Folic Acid (CAS: 59-30-3). Ruifu Chemical can provide worldwide delivery, competitive price, excellent service, small and bulk quantities available. Purchase N-(4-Aminobenzoyl)-L-Glutamic Acid, Please contact: alvin@ruifuchem.com
| Chemical Name | N-(4-Aminobenzoyl)-L-Glutamic Acid |
| Synonyms | H-4-ABZ-Glu-OH; Folic Acid Impurity A; p-Aminobenzamide Glutamic Acid; p-Aminobenzoylglutamic Acid; 4-Aminobenzoyl-L-Glutamic Acid; p-Aminobenzoyl-L-Glutamic Acid; N-(p-Aminobenzoyl)glutamic Acid; N-(p-Aminobenzoyl)-L-Glutamic Acid; Para Aminobenzoyl L-Glutamic Acid |
| CAS Number | 4271-30-1 |
| Stock Status | In Stock, Production Capacity 30 Tons per Month |
| Molecular Formula | C12H14N2O5 |
| Molecular Weight | 266.25 |
| Melting Point | 165.0~175.0℃ |
| Solubility in Hot Water | Very Faint Turbidity |
| Stability | Hygroscopic |
| COA & MSDS | Available |
| Origin of Product | China |
| Product Categories | Pharmaceutical Intermediates; Amino Acids |
| Brand | Ruifu Chemical |
| Item | Specifications | Results |
| Appearance | White or Light Yellow Crystalline Powder | Conforms |
| Identification | HPLC | Conforms |
| N-(4-Aminobenzoyl)-L-Glutamic Acid | ≥99.0% (HPLC) | 99.47% |
| Specific Rotation [α]D20 | -13.0°~-17.0° (C=2, 0.1mol/L HCl) | -15.19° |
| Melting Point | 165.0~175.0℃ | 172.0~175.0℃ |
| Moisture (K.F) | ≤0.50% | 0.35% |
| Related Substance | (by HPLC) | |
| Any Individual Impurity | ≤0.50% | <0.50% |
| Total Impurities | ≤1.00% | 0.53% |
| Heavy Metals | ≤20ppm | <10ppm |
| Infrared Spectrum | Conforms to Structure | Conforms |
| Conclusion | Complies with the Given Specifications | Conforms |
| Usage | Intermediate of Folic Acid (CAS: 59-30-3) | |
Package: Bottle, Aluminium foil bag, 25kg/Cardboard Drum, or according to customer's requirement.
Storage Condition: Keep the container tightly closed. Store in a cool, dry (2~8℃) and well-ventilated warehouse away from incompatible substances. Keep away from sunshine; avoid fire and heat sources; avoid moisture.
Shipping: Deliver to worldwide by air, by FedEx / DHL Express. Provide fast and reliable delivery.
Purity and Related substance by HPLC
Equipment:
Balance
HPLC
Reagents
Water HPLC grade
Disodium hydrogen orthophosphate
Potassium dihydrogen phosphate
ortho phosphoric acid
Methanol HPLC grade
Procedure
Preparation of buffer:
Transfer accurately weighed about 1.277gm Disodium hydrogen orthophosphate & 0.136gm Potassium dihydrogen phosphate to a500ml graduated cylinder and dissolve in 300ml of water. Dilute with water to 500ml Adjust pH 6.5 with diluted ortho phosphoric acid.
Preparation of Mobile phase:
Prepare a filtered and degassed mixture of phosphate buffer and Methanol (2:8)
Freshly Standard Preparation:
Weigh accurately about 25.0mg of the In-house working reference standard and transfer to a 25ml volumetric flask. Add 15.0ml of mobile phase to dissolve the substance and dilute with mobile phase to volume and mix. Transfer 1.0ml of above solution in to 10ml of volumetric flask and make up the volume with mobile phase and mix well.
Freshly Test Preparation:
Weigh accurately about 25.0mg of the test substance and transfer to a 25ml volumetric flask. Add 15.0ml of mobile phase to dissolve the substance and dilute with mobile phase to volume and mix. Transfer 1.0ml of above solution in to 10ml of volumetric flask and make up the volume with mobile phase and mix well.
Chromatographic System:
Column: C-18 Inertsil (ODS), 4.6mm X 25cm 5µ
Wavelength: 220nm
Flow Rate: 1.0ml/minute.
Injection Volume: 20µl each blank, standard and test preparation
Run Time: 20 minutes.
Procedures:
Inject equal volume of blank, standard and test preparation, Record the Chromatograph and measure the peak response for major peak and Unknown Peaks of test preparation.
1) Blank: Mobile Phase
2) Standard preparation : For Retention time of the in-house working reference standard
3) Test Preparation: For purity and related substance.
% Purity and impurities on chart paper basis
Risk Codes 36/37/38 - Irritating to eyes, respiratory system and skin.
Safety Description
S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
S36/37/39 - Wear suitable protective clothing, gloves and eye/face protection.
WGK Germany 3
HS Code 2922491990
Shanghai Ruifu Chemical Co., Ltd., commercial supply N-(4-Aminobenzoyl)-L-Glutamic Acid (H-4-ABZ-Glu-OH) (CAS: 4271-30-1). It is the intermediate of Folic Acid (CAS: 59-30-3). Folic Acid is a kind of B-vitamin. Folic Acid has various kinds of pharmacological and physiological effects. Folic Acid is involved in amino acid metabolism, purine and pyrimidine synthesis, and is also essential for hematopoiesis and red blood cell generation. In women pregnancy, folate can effectively prevent neural tube defects in the baby. It plays important role in fertility through contributing to spermatogenesis. Folic Acid can also reduce the incidence of heart disease, stroke and cancer. Folate deficiency may lead to various kinds of diseases including glossitis, diarrhea, depression, confusion, anemia, and fetal neural tube defectsand brain defects (during pregnancy).