Shanghai Ruifu Chemical Co., Ltd. is the leading manufacturer and supplier of L-4-Nitrophenylalanine Methyl Ester HCl (H-Phe(4-NO2)-OMe·HCl) (CAS: 17193-40-7) with high quality. Ruifu Chemical supplys a series of amino acids and derivatives. We can provide worldwide delivery, small and bulk quantities available. If you need L-4-Nitrophenylalanine Methyl Ester HCl, Please contact: alvin@ruifuchem.com
| Chemical Name | L-4-Nitrophenylalanine Methyl Ester HCl |
| Synonyms | H-Phe(4-NO2)-OMe·HCl; H-p-Nitro-Phe-OMe·HCl; L-Phe(4-NO2)-OMe·HCl; p-Nitro-L-Phe-OMe·HCl; L-4-NO2-Phe-OMe·HCl; L-4-Nitrophenylalanine Methyl Ester Hydrochloride; 4-Nitro-L-Phenylalanine Methyl Ester Hydrochloride; p-Nitrophenylalanine Methyl Ester Hydrochloride; Methyl 4-Nitro-L-Phenylalaninate Hydrochloride; (S)-(+)-4-Nitrophenylalanine Methyl Ester Hydrochloride |
| Stock Status | In Stock, Production Capacity 20 Tons per Month |
| CAS Number | 17193-40-7 |
| Molecular Formula | C10H12N2O4·HCl |
| Molecular Weight | 260.67 |
| Melting Point | 225.0~235.0℃ |
| Storage Temp. | Sealed in Dry, Store at Room Temperature |
| COA & MSDS | Available |
| Classification | Amino Acids & Derivatives |
| Brand | Ruifu Chemical |
| Risk Statements | 22-43-36 | Hazard Class | 6.1 |
| Safety Statements | 22-36/37-24/25 | Packing Group | III |
| RIDADR | 2811 | HS Code | 2922491990 |
| WGK Germany | 3 |
| Items | Inspection Standards | Results |
| Description | Off-White to Pale Yellow Powder | Conforms |
| Identification by HPLC | The retention time of the major peak in the chromatogram of the test preparation corresponds to that in the chromatogram of system suitability praparation as obtained in chromatographic purity and impurities by HPLC | Conforms |
| Specific Rotation [α]20/D | +20.0 to +24.0° (C=1, MeOH) |
+22.4° |
| Melting Point | 225.0~235.0℃ | 227.0~230.0℃ |
| Loss on Drying | <0.50% (at 105℃ for 3 Hours) | 0.23% |
| Residue on Ignition | <0.10% | 0.06% |
| Assay / Analysis Method | >98.5% (by HPLC) | 98.75% |
| Enantiomer Content | <0.15% (by HPLC) | 0.09% |
| 4-Nitro-L-Phenylalanine | <1.50% (by HPLC) | 0.91% |
| Total Impurities | <1.50% (by HPLC) | 1.25% |
| Conclusion | This Product by Inspection Accords with the Enterprise Standard | |
| Shelf Life | 24 Months In its Original Packaging if Stored Properly | |
| Main Uses | Pharmaceutical Intermediates; Zolmitriptan Intermediate | |
Package: Fluorinated Bottle, Aluminium foil bag, 25kg/Cardboard Drum, or according to customer's requirement.
Storage Condition: Store in sealed containers at cool, dry and ventilated warehouse away from incompatible substances. Protect from light and moisture.
Method of Analysis of L-4-Nitrophenylalanine Methyl Ester HCl (H-Phe(4-NO2)-OMe·HCl) (CAS: 17193-40-7)
Description: Place about 2 g of sample in a clean and dry petri-dish and observe the appearance of sample. Reporting: Report the observation
Identificaiton: Compare the retention time of the major peak in the chromatogram of the test preparation with the retention time of the major peak in the chromatogram of system suitability praparation as obtained in chromatographic purity by HPLC.
Loss on Drying: Dry a glass-stoppered, shallow weighing bottle at 105±2℃ for 30 minutes. Allow it to cool to room temperature in a desciccator. Weigh accurately the weighing bottle with stopper and record the weight (W1). Transfer about 1 to 2g accurately weighed quantity of test substance into the weighing bottle. By gentle, sidewise shaking, distribute the test specimen as evenly as practicable to a depth of about 5 mm. Weigh the loaded weighing bottle and record the weight (W2). Place the loaded bottle in the drying oven, removing the stopper and leaving it also in the chamber. Dry the test sample at 105±2℃ for 3 hours. Upon opening the chamber, close the bottle promptly, and allowe it to come to room temperature in a desiccator. Weigh accurately the weighing bottle with stopper and recoed the weight (W3). Calculate the percentage loss on drying of the sample by using following formula:
Calculation Loss on drying (% w/w)= (W2-W3) ÷ (W2-W1) x 100
Enantiomer Content by HPLC:
Chemical & Reagents:
n-Hexane AR grade
Methanol HPLC grade
Isopropyl Alcohol HPLC grade
Diethylamine AR grade
Standards: L-4-Nitrophenylalanine Methyl Ester HCl standard; L-4-Nitrophenylalanine Methyl Ester HCl enantiomer standard
Mobile Phase: Prepare a homogeneous mixture of n-Hexane, Isopropyl Alcohol, Methanol and Diethylamine (65:20:15:0.1 v/v/v/v)
Blank Preparation: Transfer 2.5ml of Methanol in a 10-ml volumetric flask, dilute to volume with mobile phase and mix.
System suitability preparation:
Weigh accurately and transfer about 5 mg each of L-4-Nitrophenylalanine Methyl Ester HCl standard and L-4-Nitrophenylalanine Methyl Ester HCl enantiomer standard in a 10-ml volumetric flask, dissolve in about 2.5ml of methanol, dilute to volume with mobile phase and mix.
Test preparation:
Weigh accurately and transfer about 5 mg of sample in a 10-mol volumetric flask, dissolve in about 2.5ml of methanol, dilute to volume with mobile phase and mix.
Instrumental parameters:
Instrument: HPLC equipped with injector, Pump, UV detector and Recorder
Column: ChiralPak-IC (4.6mm x 250mm) 5 μ or equivalent
Flow rate: 1.0 ml/minute
Wavelength: 260nm
Injection volume: 10 μL
Column coven temp.: 25℃
Run time: 20 minutes
Procedure
Inject the solutions as mentioned in the following sequence. Record the chromatograms and confirm the criteria:
Description of Injections Number of Injections Criteria
Blank Preparation 1 No interfering peak should be observed at the retention time of L-4-Nitrophenylalanine Methyl Ester HCl or enantiomer.
System suitability preparation 1 Resolution between L-4-Nitrophenylalanine Methyl Ester HCl and enantiomer peak should be not less than 2.0
Test preparation 1 Use test chromatogram to determine enantiomer content in the sample.
Relative Retention Times:
L-4-Nitrophenylalanine Methyl Ester HCl 1.0
Enantiomer About 1.11
Retention time of L-4-Nitrophenylalanine Methyl Ester HCl is about 9.8 minutes.
Calculation: In the chromatogram obtained from test preparation, disregard all peaks expect L-4-Nitrophenylalanine Methyl Ester HCl and Enantiomer. Report the enantiomer content in the sample by area normalisation method.
Chromatogrpahic Purity and Impurities by HPLC: <In House>
Chemical & Reagents:
Diammonium phosphate AR grade
Phosphoric acid AR grade
Methanol HPLC grade
Purified Water
Standards
L-4-Nitrophenylalanine Methyl Ester HCl standard
4-Nitro-L-Phenylalanine monohydrate standard
Mobile Phase-A
Dissolve 6.6 g of Diammonium phosphate in 1000ml of purified water. Adjust the pH of this solution to 6.0 with Phosphoric acid. Filter and degas. Before measuring the pH of solution, calibrate the pH meter with standard buffer solution pH 4.01 AND 7.00.
Mobile Phase-B
Methanol HPLC grade
System Suitability Preparation
Weigh accurately and transfer about 25 mg each of L-4-Nitrophenylalanine Methyl Ester HCl standard and 4-Nitro-L-Phenylalanine monohydrate standard in a 50-ml volumetric flask, dissolve in and dilute to volume with methanol and mix.
Test Preparation:
Weigh accurately and transfer about 25 mg sample in a 50-ml volumetric flask, dissolve in and dilute to volume with methanol and mix.
Instrumental parameters:
Instrument: HPLC equipped with injector, Pump, UV detector and Recorder
Column: XTerra RP-18 (4.6mm x 150mm) 5 μ or equivalent
Flow rate: 1.0 ml/minute
Wavelength: 265nm
Injection volume: 20 μL
Column coven temp.: 25℃
Run time: 45 minutes
Gradient Program
Time (min) Mobile Phase-A % Mobile Phase-B %
0.01 90 10
15 60 40
35 40 60
40 35 65
41 90 10
45 90 10
Procedure:
Inject the solutions as mentioned in the following sequence. Record the chromatograms and confirm the criteria:
Blank Preparation 1 No interfering peak should be observed at the retention time of L-4-Nitrophenylalanine Methyl Ester HCl.
System suitability preparation 1 Tailing factor for L-4-Nitrophenylalanine Methyl Ester HCl peak should be not less than 2.0
Test preparation 1 Use test chromatogram to determine chromatographic purity and impurities.
Relative Retention Times:
L-4-Nitrophenylalanine Methyl Ester HCl 1.0 (retention time about 11.4 minutes)
4-Nitro-L-Phenylalanine About 0.4
Toluene About 2.3
Calculations:
In the chromatogram obtained from test preparation, after blank correction, disregard the peak due to toluene. Report the chromatographic purity, 4-Nitro-L-Phenylalanine content and total impurities in the sample by area normalisation method.
How to Purchase? Please contact Dr. Alvin Huang: sales@ruifuchem.com or alvin@ruifuchem.com
15 Years Experience? We have more than 15 years of experience in the manufacture and export of a wide range of high quality pharmaceutical intermediates or fine chemicals.
Main Markets? Sell to domestic market, North America, Europe, India, Korea, Japanese, Australia, etc.
Advantages? Superior quality, affordable price, professional services and technical support, fast delivery.
Quality Assurance? Strict quality control system. Professional equipment for analysis include NMR, LC-MS, GC, HPLC, ICP-MS, UV, IR, OR, K.F, ROI, LOD, MP, Clarity, Solubility, Microbial limit test, etc.
Samples? Most products provide free samples for quality evaluation, shipping cost should be paid by customers.
Factory Audit? Factory audit welcome. Please make an appointment in advance.
MOQ? No MOQ. Small order is acceptable.
Delivery Time? If within stock, three days delivery guaranteed.
Transportation? By Express (FedEx, DHL), by Air, by Sea.
Documents? After sales service: COA, MOA, ROS, MSDS, etc. can be provided.
Custom Synthesis? Can provide custom synthesis services to best fit your research needs.
Payment Terms? Proforma invoice will be sent first after confirmation of order, enclosed our bank information. Payment by T/T (Telex Transfer), PayPal, Western Union, etc.
L-4-Nitrophenylalanine Methyl Ester HCl (H-Phe(4-NO2)-OMe·HCl) (CAS: 17193-40-7) is an intermediate in the synthesis of various pharmaceutical compounds. L-4-Nitrophenylalanine Methyl Ester HCl can be used for the preparation of Matijing-Su derivatives as potent anti-HBV agents. L-4-Nitrophenylalanine Methyl Ester HCl can be used as a pharmaceutical intermediate of Zolmitriptan (CAS: 139264-17-8). Zolmitriptan is a selective serotonin receptor agonist of the 1B and 1D subtypes. It is mainly used in the acute treatment of migraine attacks with or without aura and cluster headaches. Zolmitriptan takes effect through binding to human 5-HT1Band 5-HT1Dreceptors, leading to cranial blood vessel constriction and the release of sensory neuropeptides through nerve endings in the trigeminal system.