Shanghai Ruifu Chemical Co., Ltd. is the leading manufacturer and supplier of HEPPSO Hydrate (CAS: 68399-78-0) with high quality, commercial production. We can provide certificate of analysis (COA), worldwide delivery, small and bulk quantities available, strong after-sale service. Please contact: alvin@ruifuchem.com
| Chemical Name |
HEPPSO Hydrate |
| Synonyms |
HEPPSO Free Acid; 4-(2-Hydroxyethyl)piperazine-1-(2-Hydroxypropane-3-Sulfonic Acid) Hydrate; N-(Hydroxyethyl)piperazine-N'-2-Hydroxypropanesulfonic Acid; 3-[4-(2-Hydroxyethyl)-1-Piperazinyl]-2-Hydroxypropanesulfonic Acid Hydrate |
| CAS Number |
68399-78-0 |
| CAT Number |
RF-PI1677 |
| Stock Status |
In Stock, Production Scale Up to Tons |
| Molecular Formula |
C9H20N2O5S·xH2O |
| Molecular Weight |
268.33 |
| Melting Point |
158.0~160.0℃ |
| Brand |
Ruifu Chemical |
| Item |
Specifications |
| Appearance |
White Crystalline Powder |
| Purity / Analysis Method |
>99.0% (Titration) |
| Useful pH Range |
7.1~8.5 |
| pKa (25°C) |
7.5 |
| Water (by Karl Fischer) |
<7.00% |
| Heavy Metals (as Pb) |
<5ppm |
| Solubility (0.1M in H2O) |
Clear and Complete |
| pH (5% in H2O) |
7.1~8.5 |
| UV (1M in H2O) A260nm |
<0.05 Abs |
| UV (1M in H2O) A280nm |
<0.05 Abs |
| Chloride (as Cl) |
<0.005% |
| Test Standard |
Enterprise Standard |
| Infrared Spectrum |
Conforms to Structure |
| NMR |
Conforms to Structure |
| Grade |
Ultra Pure Grade; Molecular Biology Grade |
| Usage |
Biological Buffer; Zwitterionic Buffer
|
Package: Bottle, Aluminium foil bag, 25kg/Cardboard Drum, or according to customer's requirement.
Storage Condition: Store in sealed containers at cool and dry place; Protect from light and moisture.
HEPPSO Hydrate (CAS: 68399-78-0) is a zwitterionic buffer. The working pH range of HEPPSO buffer is 7.1~8.5. HEPPSO is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is commonly used as an ampholytic separator to create a pH gradient in isoelectric focusing. HEPPSO displays no toxicity for crustaceans with which it has been studied and has been deemed suitable for toxicological studies. This buffer is capable of forming radicals and is therefore not suitable for redox reactions. HEPPSO forms a complex with Cu(II) ions so stability constants and concentrations should be taken into consideration when using this buffer in a solution containing copper ions. Though HEPPSO binds to coppers ions, it is still suitable for use with the bicinchoninic acid (BCA) assay. Diminished protein-induced color development can be accounted for or even eliminated by precipitating protein prior to reaction with bicinchoninic acid.