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HEPES CAS 7365-45-9
HEPPS CAS 16052-06-5
| Chemical Name | HEPES |
| Synonyms | HEPES Free Acid; 2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic Acid |
| CAS Number | 7365-45-9 |
| CAT Number | RF-PI1629 |
| Stock Status | In Stock, Production Scale Up to Tons |
| Molecular Formula | C8H18N2O4S |
| Molecular Weight | 238.30 |
| Melting Point | 234.0~238.0℃ |
| Density | 1.07 g/mL at 20℃ |
| Solubility in Water | Almost Transparency |
| Brand | Ruifu Chemical |
| Item | Specifications |
| Grade | Ultra Pure Grade |
| Appearance | White Crystalline Powder |
| Purity | >99.5% (Titration, Dry Basis) |
| Water (by Karl Fischer) | <0.50% |
| Loss on Drying | <0.50% (104℃, 4h) |
| Residue on Ignition | <0.10% |
| Insoluble Matter | Passes Filter Test |
| Solubility (1M in H2O) | Clear and Complete |
| Heavy Metals (as Pb) | <5ppm |
| Iron (Fe) | <0.0005% |
| Chloride (CI) | <0.005% |
| Sulfate (SO4) | <0.005% |
| Phosphate (P) | <0.001% |
| Arsenic (As) | <0.0001% |
| Lead (Pb) | <0.001% |
| Cu | <5ppm |
| Absorbance 250nm | <0.05 (1M aq.) |
| Absorbance 260nm | <0.05 (1M aq.) |
| Absorbance 280nm | <0.05 (1M aq.) |
| Useful pH Range | 6.8~8.2 (1% in H2O) |
| pKa (25℃) | 7.35~7.69 |
| Endotoxin | <0.1EU/ml (0.2% Solution) |
| Infrared Spectrum | Conforms to Structure |
| Test Standard | Enterprise Standard |
| Usage | Biological Buffer; Good's Buffer Component for Biological Research |
Package: Bottle, Aluminium foil bag, 25kg/Cardboard Drum, or according to customer's requirement.
Storage Condition: Store in sealed containers at cool and dry place; Protect from light and moisture.
HEPES (CAS: 7365-45-9) is common buffer for biological sciences, particularly used in cell culture to maintain physiological pH. It acts a buffering component, which is used in the preparation of buffers. It is described as one of the best all-purpose buffers available for use in biological research. HEPES has been used as a component of: Hank′s balanced salt solution, Dulbecco′s modified eagle′s medium and no-glucose DMEM, which are used for the preparation of tissue slices. Buffers used in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity.