L-Glutathione Reduced CAS 70-18-8 Assay 98.0~101.0% (Titration)

Shanghai Ruifu Chemical Co., Ltd. is the leading manufacturer of Glutathione Reduced Form (GSH) (CAS: 70-18-8) with high quality. Ruifu Chemical can provide worldwide delivery, competitive price, excellent service, small and bulk quantities available. Purchase Glutathione Reduced Form, Please contact: alvin@ruifuchem.com

Package: Fluorinated Bottle, Aluminium foil bag, 25kg/Cardboard Drum, or according to customer's requirement.
Storage Condition: Keep the container tightly closed and store in a cool, dry and well-ventilated warehouse away from incompatible substances. Protect from light and moisture.
Shipping: Deliver to worldwide by air, by FedEx / DHL Express. Provide fast and reliable delivery. 

Glutathione
C10H17N3O6S 307.32
Pentanoic acid, 2-amino-5-[(R)-1-(carboxymethylamino)-3-mercapto-1-oxopropan-2-ylamino]-5-oxo, (S);
N-(N-l--Glutamyl-l-cysteinyl)glycine [70-18-8].
DEFINITION
Glutathione contains NLT 98.0% and NMT 101.0% of C10H17N3O6S, as glutathione, calculated on the dried basis.
IDENTIFICATION
• A. Infrared Absorption <197K>
• B. Optical Rotation, Specific Rotation <781S>
Sample solution: 40 mg/mL in water
Acceptance criteria: 15.5° to 17.5°, at 20℃
ASSAY
• Procedure
Sample: 500 mg of glutathione previously dried
Titrimetric system
(See Titrimetry <541>.)
Mode: Direct titration
Titrant: 0.1 N iodine VS
Endpoint detection: Visual
Blank: 50 mL of metaphosphoric acid (1 in 50)
Analysis: Dissolve the Sample in 50 mL of metaphosphoric acid (1 in 50) and titrate with the Titrant.
Calculate the percentage of glutathione (C10H17N3O6S) in the portion of Glutathione taken:
Result = [(V B) × N × F × 100]/W
V = titrant volume of the Sample (mL)
B = titrant volume of the Blank (mL)
N = titrant normality (mEq/mL)
F = equivalency factor, 307.32 mg/mEq
W = weight of the Sample (mg)
Acceptance criteria: 98.0%~101.0% on the dried basis
IMPURITIES
Inorganic Impurities
• Ammonium
Standard solution: 10 µg of ammonium from a diluted ammonium chloride solution
Sample solution: 50 mg of Glutathione
Analysis: Transfer the Sample solution and the Standard solution to separate 25-mL jars fitted with caps, and dissolve in 1 mL of water. Add 0.30 g of magnesium oxide. Close immediately after placing a piece of silver manganese paper 5-mm square, wetted with a few drops of water, under the caps. Swirl, avoiding projections of liquid, and allow to stand at 40 for 30 min.
Acceptance criteria: If the silver manganese paper shows a gray color, it is not more intense than the standard (NMT 200 ppm).
• Arsenic <211>: NMT 2 ppm
• Chloride and Sulfate, Chloride <221>: Dissolve 0.7 g with water to make 15 mL. The solution shows no more chloride than corresponds to 0.20 mL of 0.020 N hydrochloric acid (NMT 200 ppm).
• Chloride and Sulfate, Sulfate 221: Dissolve 0.8 g in water to make 15 mL. The solution shows no more sulfate than corresponds to 0.25 mL of 0.020 N sulfuric acid (NMT 300 ppm).
• Heavy Metals, Method I <231>: NMT 10 ppm
• Iron <241>: NMT 10 ppm
• Residue on Ignition <281>: NMT 0.1%
Organic Impurities
• Procedure
Mobile phase: 6.8 g/L of potassium dihydrogen phosphate with 2.02 g/L of sodium 1-heptane sulfonate. Adjust with phosphoric acid to a pH of 3.0. Mix 970 mL of this solution with 30 mL of methanol.
System suitability solution: 0.1 mg/mL of USP l-Phenylalanine RS, 0.5 mg/mL of USP Glutathione RS, and 0.5 mg/mL of USP Ascorbic acid RS in Mobile phase
Standard solution: 0.01 mg/mL of USP Glutathione RS in Mobile phase. [Note—This solution has a concentration equivalent to 2.0% of that of the Sample solution. ]
Sample solution: 50 mg of glutathione in 100 mL of Mobile phase. [Note—Allow the solution to stand for 5 min before use. ]
Chromatographic system
(See Chromatography <621>, System Suitability.)
Mode: LC
Detector: UV 210 nm
Column: 4.6-mm × 15-cm; 5-µm packing L1
Column temperature: 30
Flow rate: Adjust so that the retention time of glutathione is about 5 min.
Injection size: 10 µL
System suitability
Sample: System suitability solution
Suitability requirements
Resolution: NLT 5.0 between the ascorbic acid and glutathione peaks; and NLT 5.0 between the glutathione and l-phenylalanine peaks
Relative standard deviation: NMT 1.5% for replicate injections
Analysis
Samples: Standard solution and Sample solution
Calculate the percentage of any impurity in the portion of Glutathione taken:
Result = (rU/rS) × (CS/CU) × 100
rU = peak response of any peak from the Sample solution other than glutathione
rS = peak response of the glutathione peak from the Standard solution
CS = concentration of USP Glutathione RS in the Standard solution (mg/mL)
CU = concentration of Glutathione in the Sample solution (mg/mL)
Acceptance criteria
Individual impurity: NMT 1.5% for the impurity with the relative retention time of about 4
Total impurities: NMT 2.0%
SPECIFIC TESTS
• Clarity and Color of Solution
Sample solution: 0.1 g/mL in water
Analysis: Using identical tubes of colorless, transparent, neutral glass with a flat base and an internal diameter of 15-25 mm, compare the liquid to be examined with water, the depth of the layer being 40 mm. Compare the colors in diffused daylight, viewing vertically against a white background.
Acceptance criteria: The solution is clear and colorless.
• Loss on Drying <731>: NMT 0.5%, 105℃ for 3 h
ADDITIONAL REQUIREMENTS
• Packaging and Storage: Preserve in tight containers.
• USP Reference Standards <11>
USP Ascorbic Acid RS
USP Glutathione RS
USP l-Phenylalanine RS

How to Purchase? Please contact Dr. Alvin Huang: sales@ruifuchem.com or alvin@ruifuchem.com 

15 Years Experience? We have more than 15 years of experience in the manufacture and export of a wide range of high quality pharmaceutical intermediates or fine chemicals.

Main Markets? Sell to domestic market, North America, Europe, India, Korea, Japanese, Australia, etc.

Advantages? Superior quality, affordable price, professional services and technical support, fast delivery.

Quality Assurance? Strict quality control system. Professional equipment for analysis include NMR, LC-MS, GC, HPLC, ICP-MS, UV, IR, OR, K.F, ROI, LOD, MP, Clarity, Solubility, Microbial limit test, etc.

Samples? Most products provide free samples for quality evaluation, shipping cost should be paid by customers.

Factory Audit? Factory audit welcome. Please make an appointment in advance.

MOQ? No MOQ. Small order is acceptable.

Delivery Time? If within stock, three days delivery guaranteed.

Transportation? By Express (FedEx, DHL), by Air, by Sea.

Documents? After sales service: COA, MOA, ROS, MSDS, etc. can be provided.

Custom Synthesis? Can provide custom synthesis services to best fit your research needs.

Payment Terms? Proforma invoice will be sent first after confirmation of order, enclosed our bank information. Payment by T/T (Telex Transfer), PayPal, Western Union, etc. 

Hazard Symbols Xi - Irritant
Risk Codes R68 - Possible risk of irreversible effects
R36/37/38 - Irritating to eyes, respiratory system and skin.
Safety Description S24/25 - Avoid contact with skin and eyes.
S36/37/39 - Wear suitable protective clothing, gloves and eye/face protection.
S27 - Take off immediately all contaminated clothing.
S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
WGK Germany 2
RTECS MC0556000
FLUKA BRAND F CODES 9-23
TSCA Yes
HS Code 2930909099

Glutathione Reduced Form (GSH) (CAS: 70-18-8) is a tripeptide which consists of glutamic acid, cysteine, and glycin. The L-Glutathione Enriched Yeast obtained via microbial fermentation, then obtain the L-Glutathione Reduced by modern technology's separation and purification .It is an important functional factor,which has many functions, such as anti-oxidant, free radical scavenging, detoxification, enhance immunity, anti-aging, anti-cancer, anti-radiation hazards and others.
1. L-Glutathione Role of Clinical Treatment and Prevention
Under pathological conditions when endogenous GSH reduction, timely exogenous GSH has become to be. Exogenous GSH supplement can prevent and treat related diseases, maintain a healthy body.
(1) Radiation sickness and radiation safety: radiation, radioactive substances or because of leukopenia caused by anticancer drugs and other symptoms can play a protective effect.
(2) To protect the liver, detoxification, inactivation of hormones, and to promote bile acid metabolism and help absorb fat and fat-soluble vitamins digestive tract.
(3) Anti-allergy, or inflammation caused by hypoxemia in patients with systemic or local, can reduce cell damage and promote repair.
(4) To improve the course of certain diseases and symptoms as adjuvant drugs. Such as: hepatitis, hemolytic disease, and keratitis, cataract and retinal diseases, such as eye disease and improve vision.
(5) Easy to speed up the metabolism of acids in the excretion of free radicals, which play a beauty skin care, anti-aging effect.
2. Food Additives
(1) L-Glutathione reduced added to pasta, to make manufacturers reduce the time of bread to the original one-half or one third, and serve to strengthen the role of food nutrition and other features.
(2) To add to the yogurt and baby food, the equivalent of vitamin C, can play a stabilizing agent.
(3) In its mix to the surimi to prevent color deepened.
(4) To the meat and cheese and other foods, have enhanced the effect of flavor.
3. Beauty Products / Cosmetics
Prevent the infiltration of Los tyrosinase in order to achieve the purpose of inhibiting melanin formation. On eliminate wrinkles, increase skin elasticity, shrink pores, lighten pigment, the body has an excellent whitening effect. Glutathione as the main ingredient in cosmetic products in Europe and the United States was welcomed by the decades.
Beauty and personal care: Glutathione can eliminate wrinkles, increase skin elasticity, shrink pores, reduce pigment, the body has an excellent whitening effect.